Mark Paddock
Publications: 1987-current [Back to home page]

1. Characterization of Three Herbicide Resistant Mutants of Rhodopseudomonas sphaeroides 2.4.1: Structure - Function Relationship; M. L. Paddock, J.C. Williams, S.H. Rongey, E.C. Abresch, G. Feher and M.Y. Okamura; Progress in Photosynthesis Research, Vol. 3, pp. III.11.775-III.11.778 (Proceedings of the VIIth International Conference on Photosynthesis, Brown University, August 1986, Ed. J. Biggins), Martinus Nijhoff, Boston, 1987.

2. Reaction Centers from Three Herbicide Resistant Mutants of Rhodobacter sphaeroides 2.4.1: Sequence Analysis and Preliminary Characterization; M.L. Paddock, S.H. Rongey, E.C. Abresch, G. Feher and M.Y. Okamura; Photosynthesis Research 17, 75-96 (1988).

3. Pathway of Proton Transfer in Bacterial Reaction Centers: Replacement of Glutamic Acid 212 in the L Subunit by Glutamine Inhibits Quinone (Secondary Acceptor) Turnover; M.L. Paddock, S.H. Rongey, G. Feher and M.Y. Okamura; Proc. Natl. Acad. Sci. USA , 86, 6602-6606 (1989).

4. Protonation of Quinones in Reaction Centers from Rb. Sphaeroides; G. Feher, P.H. McPherson, M.L. Paddock, S.H. Rongey, M. Schönfeld and M.Y. Okamura; in Current Research in Photosynthesis, Vol. I, pp. I.1.39-I.1.46 (Proceedings of the VIIIth International Conference on Photosynthesis, Stockholm, Sweden, August l989, Ed. M. Baltscheffsky), Kluwer Academic Publishers, Dordrecht, 1990.

5. Site-Directed Mutagenesis of Reaction Centers from Rhodobacter sphaeroides; M.L. Paddock, S.H. Rongey, J.W. Farchaus, G. Feher and M.Y. Okamura; in Current Research in Photosynthesis, Vol. I, pp. I.1.161-I.1.164 (Proceedings of the VIIIth International Conference on Photosynthesis, Stockholm, Sweden, August l989, Ed. M. Baltscheffsky), Kluwer Academic Publishers, Dordrecht, 1990.

6. Pathway of Proton Transfer in Bacterial Reaction Centers: Replacement of Serine-L223 by Alanine Inhibits Electron and Proton Transfers Associated with the Reduction of Quinone to Dihydroquinone; M.L. Paddock, P.H. McPherson, G. Feher and M.Y. Okamura; Proc. Natl. Acad. Sci., 87, 6803-6807 (1990).

7. Reaction Centers from three Herbicide Resistant Mutants of Rhodobacter sphaeroides 2.4.1: Kinetics of Electron Transfer Reactions; M.L. Paddock, G. Feher and M.Y. Okamura; Photosynthesis Research 27, 109-119 (1991).

8. Proton Transfer Pathways in Photosynthetic Reaction Centers Studied by Site-Directed Mutagenesis (1992); G. Feher, M. L. Paddock, S.H. Rongey and M.Y. Okamura; in Membrane Proteins: Structure, Interactions and Models, A. Pullman, J. Jortner, B. Pullman, eds.; Kluwer Academic Publishers, Dordrecht, Vol. 125, pp. 481-495.

9. Proton Transfer in Bacterial Reaction Centers: Second Site Mutations Asn M44 to Asp or Arg M233 to Cys Restore Photosynthetic Competence to Asp L213 to Asn Mutants in RCs from Rb. Sphaeroides; M.Y. Okamura, M. L. Paddock, P.H. McPherson, S. Rongey and G. Feher; in Research in Photosynthesis, Vol. I, pp. I.3.349-I.3.356 (Proceedings of the IXth International Congress on Photosynthesis, Nagoya, Japan, August 1992, Ed. N. Murata), Kluwer Academic Publishers, the Netherlands, 1992.

10. Proton Transfer Mutants of Rb. sphaeroides: Characterization of Reaction Centers by Infrared Spectroscopy; R. Hienerwadel, E. Nabedryk, M. L. Paddock, S. Rongey, M.Y. Okamura, W. Mantele and J. Breton; in Research in Photosynthesis, Vol. I, pp. I.3.437-I.3.440 (Proceedings of the IXth International Congress on Photosynthesis, Nagoya, Japan, August 1992, Ed. N. Murata), Kluwer Academic Publishers, the Netherlands, 1992.

11. Pathway of Proton Transfer in Bacterial Reaction Centers: Second Site Mutation Asn-M44 to Asp Restores Electron and Proton Transfer in Reaction Centers from the Photosynthetically Deficient Asp-L213 to Asn Mutant of Rhodobacter sphaeroides; S.H. Rongey, M.L. Paddock, G. Feher and M.Y. Okamura; Proc. Natl. Acad. Sci. 90, 1325-1329 (1993).

12. Pathway of Proton Transfer in Bacterial Reaction Centers: The Role of Asp-L213 in Proton Transfers Associated with the Reduction of Quinone to Dihydroquinone; M.L. Paddock, S.H. Rongey, P.H. McPherson, A. Juth, G. Feher and M.Y. Okamura; Biochemistry 33, 734-745 (1994).

13. Protonation and Free Energy Changes Associated with Formation of Q_B H₂ in Native and Glu-L212 to Gln Mutant Reaction Centers from Rhodobacter sphaeroides; P.H. McPherson, M. Schönfeld, M.L. Paddock, M.Y. Okamura and G. Feher; Biochemistry 33, 1181-1193 (1994).

14. Crystallographic Analyses of Site-Directed Mutants of the Photosynthetic Reaction Center from Rhodobacter sphaeroides; A.J. Chirino, E.J. Lous, M. Huber, J.P. Allen, C.C. Schenck, M.L. Paddock, G. Feher and D.C. Rees; Biochemistry 33, 4584-4593 (1994).

15. Direct Charge Recombination from D⁺Q_AQ_B^- to DQ_AQ_B in Bacterial Reaction Centers from Rhodobacter sphaeroides ; A. Labahn, M.L. Paddock, P.H. McPherson, M.Y. Okamura and G. Feher; J. Phys. Chem. 98, 3417-3423 (1994).

16. Protonation of Glu L212 Following Q_B^- Formation in the Photosynthetic Reaction Center of Rhodobacter sphaeroides: Direct Evidence from Time-Resolved Infrared Spectroscopy; R. Hienerwadel, S. Grzybek, C. Fogel, W. Kreutz, M.Y. Okamura, M.L. Paddock, G. Feher, J. Breton, E. Nabedryk and W. Mantele; Biochemistry 34, 2832-2843 (1995).

17. Fourier Transform Infrared Difference Spectroscopy of Secondary Quinone Acceptor Photoreduction in Proton Transfer Mutants of Rhodobacter sphaeroides; E. Nabedryk, J. Breton, R. Hienerwadel, C. Fogel, W. Mantele, M.L. Paddock and M.Y. Okamura; Biochemistry 34, 14722-14732 (1995).

18. Pathway of Proton Transfer in Bacterial Reaction Centers: Further Investigations on the Role of SER- L223 Studied by Site-Directed Mutagenesis; M.L. Paddock, G. Feher and M.Y. Okamura; Biochemistry 34, 15742-15750 (1995).

19. Suppressor Mutation in the H-Subunit Partially Restores Proton Transfer in Asp-L213 to Asn Mutant Reaction Centers from Rhodobacter sphaeroides; M.E. Senft, M.L. Paddock, S.H. Rongey, G. Feher and M.Y. Okamura; in Photosynthesis: from Light to Biosphere, Vol. 1, pp. 639-642 (Proceedings of the Xth International Photosynthesis Congress, in Montpellier, France, August 20-25, 1995, Ed. P. Mathis), Kluwer Academic Publishers, Dordrecht, 1995.

20. Asymmetry of the Binding Sites of Q_A^- and Q_B^- in Reaction Centers of Rb. sphaeroides Probed by Q-Band EPR with ¹³C-Labeled Quinones; R. A. Isaacson, E. C. Abresch, F. Lendzian, C. Boullais, M. L. Paddock, C. Mioskowski, W. Lubitz and G. Feher; in Structure and Dynamics, M. E. Beyerle, editor, (Springer-Verlag, Berlin) pp 353-368 (Presented at Feldafing Meeting, March 1-4, 1995, Feldafing, Germany).

21. Mechanism of Proton-Coupled Electron Transfer for Quinone (Q_B) Reduction in Reaction Centers of Rb. sphaeroides; M. S. Graige, M.L. Paddock, J.M Bruce, G. Feher and M.Y. Okamura; J. Am. Chem. Soc. 118, 9005-9016 (1996).

22. Light-Induced Electrogenic Events Associated with Proton Uptake Upon Forming Q_B^- in Bacterial Wild-Type and Mutant Reaction Centers; P. Brzezinski, M.L. Paddock, M.Y. Okamura and G. Feher; Biochim. Biophys. Acta, 1321, 149-156 (1997).

22. Proton and Electron Transfer to the Secondary Quinone (Q_B) in Bacterial Reaction Centers: The Effect of changing the Electrostatics in the Vicinity of Q_B by interchanging Asp and Glu at the L212 and L213 Sites; M.L. Paddock, G. Feher and M.Y. Okamura; Biochemistry 36, 14238-14249 (1997).

23. Characterization of Second Site Mutations Show that Fast Proton Transfer to Q_B^- is Restored in Bacterial Reaction Centers of Rhodobacter sphaeroides Containing the Asp-L213 to Asn Lesion; M.L. Paddock, M.E. Senft, M.S. Graige, S.H. Rongey, T. Turanchik, G. Feher and M.Y. Okamura; Photosynthesis Research 55, 281-291 (1998).

25. Identification of Proton Transfer Pathways in the X-Ray Crystal Structure of the Bacterial Reaction Center from Rhodobacter sphaeroides; E.C. Abresch, M.L. Paddock, M.H.B. Stowell, T.M. McPhillips, H.L. Axelrod, S.M. Soltis, D.C. Rees, M.Y. Okamura and G. Feher; Photosynthesis Research 55, 119-125 (1998).

26. Free Energy Dependence of the Direct Charge Recombination from the Primary and Secondary Quinones in Reaction Centers from Rhodobacter sphaeroides; J.P. Allen, J. C. Williams, M. Graige, M. L. Paddock, A. Labahn, G. Feher and M.Y. Okamura; Photosynthesis Research 55, 227-233 (1998).

27. Direct Evidence of Structural Changes in Reaction Centers of Rb. sphaeroides Containing Suppressor Mutations for Asp L213 to Asn: A FTIR Study of Q_B Photoreduction; E. Nabedryk, J. Breton, M.Y. Okamura and M.L. Paddock; Photosynthesis Research 55, 293-299 (1998).

28. A puhA Gene Deletion and Plasmid Complementation System for Facile Site-Directed Mutagenesis Studies of Reaction Center H Protein Function in R. sphaeroides; X-Y Chen, V. Yurkov, M. L. Paddock, M. Y. Okamura and J. T. Beatty; Photosynthesis Research 55, 369-373 (1998).

29. Proton Uptake by Carboxylic Acid Groups upon Photoreduction of the Secondary Quinone (Q_B) in Bacterial Reaction Centers from Rhodobacter Sphaeroides: FTIR Studies on the Effects of Replacing Glu H173; E. Nabedryk, J. Breton, M. Y. Okamura and M. L. Paddock; Biochemistry 37, 14457-14462 (1998).

30. Identification of the Proton Pathway in Bacterial Reaction Centers: Inhibition of Proton Transfer by Binding of Zn²⁺ or Cd²⁺; M. L. Paddock, M. S. Graige, G. Feher & M. Y. Okamura; Proc. Natl. Acad. Sci. USA 96, 6183-6188 (1999).

31. Observation of the Protonated Semiquinone Intermediate in Isolated Reaction Centers from Rb. sphaeroides: Implications for the Mechanism of Electron & Proton Transfer in Proteins; M.S. Graige, M.L. Paddock, G. Feher, and M.Y. Okamura: Biochemistry, 38,11465-11473 (1999).

32. Determination of the Binding Sites of the Proton Transfer Inhibitors Cd²⁺ and Zn²⁺ in Bacterial Reaction Centers; H.L. Axelrod, E.C. Abresch, M.L. Paddock, M.Y. Okamura and G. Feher: Proc. Natl. Acad. Sci., USA 97, 1542-1547 (2000).

33. Identification of the Proton Pathway in Bacterial Reaction Centers: Replacement of Asp-M17 and Asp-L210 with Asn Reduces the Proton Transfer Rate in the Presence of Cd²⁺; M.L. Paddock, G. Feher and M.Y. Okamura, Proc. Natl. Acad. Sci., USA 97, 1548-1553 (2000).

34. Proton and Electron Transfer in Bacterial Reaction Centers; M.Y. Okamura, M.L. Paddock, M.S. Graige and G. Feher, Biochim. Biophys. Acta 1458, 148-163 (2000).

35. EPR Study of the Molecular and Electronic Stucture of the Semiquinone Biradical Q_A^- Q_B^- in Photosynthetic Reaction Centers from Rb. sphaeroides; R. Calvo, E.C. Abresch, R. Bittl, G. Feher, W. Hofbauer, R.A. Isaacson, W. Lubitz, M.Y. Okamura, and M.L. Paddock; J. Am. Chem. Soc. 122, 7327-7341 (2000).

36. Identification of the Proton Pathway in Bacterial Reaction Centers: Both Protons Associated with Reduction of Q_B to Q_BH₂ Share a Common Entry Point; P. Ädelroth, M.L. Paddock, L.B. Sagle, G. Feher and M.Y. Okamura; Proc. Natl. Acad. Sci., USA, 97, 13086-13091 (2000).

37 Effect of Metal Binding on Electrogenic Proton Transfer Associated with Reduction of the Secondary Electron Acceptor (Q_B) in Rhodobacter sphaeroides Chromatophores; S. Keller, J.T. Beatty, M. Paddock, J. Breton and W. Leibl; Biochemistry, 40, 429-439 (2001).

38. EPR Study of the Semiquinone Biradical Q_A^-Q_B^-. in Photosynthetic Reaction Centers from Rb. Sphaeroides at 326 GHz: Determination of the Exchange Interaction J₀; R. Calvo, R.A. Isaacson, M.L. Paddock, E.C. Abresch, M.Y. Okamura, A.-L. Maniero, L.-C. Brunel and G. Feher; Journal of Physical Chemistry B, 105, 4053-4057 (2001).

39. Identification of the Proton Pathway in Bacterial Reaction Centers: Cooperation between Asp-M17 and Asp-L210 Facilitates Proton Transfer to the Secondary Quinone (Q_B); M.L. Paddock, P. Ädelroth, C. Chang, E.C. Abresch, G. Feher and M.Y. Okamura; Biochemistry, 40, 6893-6902 (2001).

40. Identification of the Proton Pathway in Bacterial Reaction Centers: Decrease of Proton Transfer Rate by Mutation of surface Histidines at H126 and H128 and Chemical Rescue by Imidazole Identifies the Initial Proton Donors; P. Ädelroth, M.L. Paddock, A. Tehrani, J.T. Beatty, G. Feher and M.Y. Okamura; Biochemistry, 40, 14538-14546 (2001).

41. Rapid-scan Fourier Transfor Infrared Spectroscopy shows Coupling of Glu-L212 Protonation and Electron to Q_B in Rhodobacter sphaeroides Reaction Centers; A. Mezzetti, E. Nabedryk, J. Breton, M.Y. Okamura, M.L. Paddock, G. Giacomett, W. Leible; BBA – Bioenergetics, 1553, 320-330 (2002).

42. Determination of Proton Transfer Rates by Chemical Rescue: Application to Bacterial Reaction Centers; M.L. Paddock, P. Ädelroth, J.T. Beatty, G. Feher, M.Y. Okamura; Biochemistry, 41, 14716-14725 (2002).