Melvin Okamura Publications 1990-current: [Back to home page]
55. Protonation of Quinones in Reaction Centers from Rb. Sphaeroides; G.
Feher, P. H. McPherson, M. Paddock, S. H. Rongey, M. Schönfeld and M. Y. Okamura; in Current Research in
Photosynthesis, Vol. I, pp. I.1.39-I.1.46 (Proceedings of
the VIIIth International Conference on
Photosynthesis,
56. Site-Directed Mutagenesis of Reaction
Centers from Rhodobacter sphaeroides; M. L. Paddock, S. H. Rongey,
J. W. Farchaus, G. Feher and M. Y. Okamura; in Current
Research in Photosynthesis, Vol. I, pp. I.1.161-I.1.164
(Proceedings of the VIIIth International Conference
on Photosynthesis,
57. FTIR Studies of the D+
QA-· and D+
QB-· States in Reaction Centers from Rb. Sphaeroides; K.
A. Bagley, E. Abresch, M. Y. Okamura and G. Feher, M.
Bauscher and W. Mantele, E.
Nabedryk and J. Breton; in Current Research in
Photosynthesis, Vol. I, pp. I.1.77-I.1.80 (Proceedings of
the VIIIth International Conference on
Photosynthesis,
58. ENDOR Studies of the Intermediate Electron Acceptor Radical Anion
I - in Reaction Centers
of Rps. Viridis ;
W. Lubitz, B. Bonigk, M.
Plato, R. A. Isaacson, M. Y. Okamura and G. Feher; in Current Research in
Photosynthesis, Vol. I, pp. I.1.141-I.1.144 (Proceedings
of the VIIIth International Conference on
Photosynthesis,
59. Electron Transfer from the
60. EPR Investigation of Photosynthetic Reaction Centers from Rb. Sphaeroides R-26 in which Fe2+ was Replaced by Cu2+:Determination of Hyperfine Interactions and Exchange and Dipole-Dipole Interactions Between Cu2+ and QA-· ; R. Calvo, M. C. G. Passeggi, R. A. Isaacson, M. Y. Okamura and G. Feher; Biophysical J., 58, 149-165 (1990).
61. pH-Dependence of the Free Energy Gap Between DQA and D+ QA-· Determined from Delayed Fluorescence in Reaction Centers from Rhodobacter Sphaeroides; P. H. McPherson, V. Nagarajan, W. W. Parson, M. Y. Okamura and G. Feher; Biochim. Biophys. Acta, 1019, 91-94 (1990).
62. Pathway of Proton Transfer in Bacterial Reaction Centers: Replacement of Serine-L223 by Alanine Inhibits Electron and Proton Transfers Associated with the Reduction of Quinone to Dihydroquinone; M. L. Paddock, P. H. McPherson, G. Feher and M. Y. Okamura; Proc. Natl. Acad. Sci., 87, 6803-6807 (1990).
63. Reaction Centers from three Herbicide Resistant Mutants of Rhodobacter sphaeroides 2.4.1: Kinetics of Electron Transfer Reactions; M. L. Paddock, G. Feher and M. Y. Okamura; Photosynthesis Research 27, 109-119 (1991).
64. Reaction of cytochrome c and c2 with the Rhodobacter sphaeroides Reaction Center Involves the Heme Crevice Domain; J. Hall, X. Zha, B. Durham, P. O'Brien, B. Viera, D. Davis, M. Okamura and F. Millet; Biochemistry 26, 4494-4500 (1987).
65. Protonation of Interacting Residues in a Protein by a Monte Carlo Method: Application to Lysozyme and the Photosynthetic Reaction Center of Rhodobacter sphaeroides; P. Beroza, D. R. Fredkin, M. Y. Okamura and G. Feher; Proc. Natl. Acad. Sci., 88, 5804-5808 (1991).
66. Proton Transfer in Reaction Centers from Photosynthetic Bacteria; M. Y. Okamura and G. Feher; Ann. Rev. Biochem., 61, 861-896 (1992).
67. Proton Transfer Pathways in Photosynthetic Reaction Centers Studied by Site-Directed Mutagenesis (1992); G. Feher, M. L. Paddock, S.H. Rongey and M.Y. Okamura; in Membrane Proteins: Structure, Interactions and Models, A. Pullman, J. Jortner, B. Pullman, eds.; Kluwer Academic Publishers, Dordrecht, Vol. 125, pp. 481-495.
68. Structural Changes Following the Formation of D+ QA-· in Bacterial Reaction Centers: Measurement of Light-Induced Electrogenic Events in RCs Incorporated in a Phospholipid Monolayer; Peter Brzezinski, Melvin Y. Okamura and George Feher (1992); in The Photosynthetic Bacterial Reaction Center II, J. Breton and A. Vermeglio, editors; Plenum Press, Publishers, pp. 321-330. (Presented at the Conference on Structure, Function and Dynamics of the Bacterial Reaction Center, in Cadarache, France, May 1992).
69. Proton Transfer Pathways in the Reaction Center of Rhodobacter sphaeroides: A Computational Study; P. Beroza, D. R. Fredkin, M.Y. Okamura and G. Feher (1992); in The Photosynthetic Bacterial Reaction Center II , J. Breton and A. Vermeglio, editors; Plenum Press, Publishers, pp. 363-374. (Presented at the Conference on Structure, Function and Dynamics of the Bacterial Reaction Center, in Cadarache, France, May 1992).
70. Proton Transfer in Bacterial Reaction
Centers: Second Site Mutations Asn M44 to Asp
or Arg M233 to Cys Restore
Photosynthetic Competence to Asp L213 to Asn Mutants
in RCs from Rb. Sphaeroides; M.Y. Okamura, M. L. Paddock, P.H. McPherson,
S. Rongey and G. Feher; in Research in
Photosynthesis, Vol. I, pp. I.3.349-I.3.356 (Proceedings of
the IXth International Congress on Photosynthesis,
Nagoya, Japan, August 1992, Ed. N. Murata), Kluwer
Academic Publishers, the
71. Crystallization of the PSII-Reaction Center; N. Adir, M.Y. Okamura and G. Feher; in Research in Photosynthesis, Vol. II, pp. II.5.195-II.5.198 (Proceedings of the IXth International Congress on Photosynthesis, Nagoya, Japan, August 1992, Ed. N. Murata), Kluwer Academic Publishers, the Netherlands, 1992.
72. Proton Transfer Mutants of Rb. sphaeroides:
Characterization of Reaction Centers by Infrared Spectroscopy; R. Hienerwadel, E. Nabedryk, M. L.
Paddock, S. Rongey, M. Y. Okamura, W. Mantele and J. Breton; in Research in Photosynthesis,
Vol. I, pp. I.3.437-I.3.440 (Proceedings of the IXth International Congress on Photosynthesis, Nagoya,
Japan, August 1992, Ed. N. Murata), Kluwer Academic
Publishers, the
73. Pathway of Proton Transfer in Bacterial Reaction Centers: Second Site Mutation Asn-M44 to Asp Restores Electron and Proton Transfer in Reaction Centers from the Photosynthetically Deficient Asp-L213 to Asn Mutant of Rhodobacter sphaeroides; S.H. Rongey, M. L. Paddock, G. Feher and M.Y. Okamura; Proc. Natl. Acad. Sci. 90, 1325-1329 (1993).
74. Light-Induced Proton Uptake by Photosynthetic Reaction Centers from Rhodobacter sphaeroides R-26, II. Protonation of the State DQAQB2- ; P.H. McPherson, M.Y. Okamura and G. Feher; Biochim. Biophys. Acta 1144, 309-324 (1993).
75. Pathway of Proton Transfer in Bacterial Reaction Centers: The Role of Asp-L213 in Proton Transfers Associated with the Reduction of Quinone to Dihydroquinone; M. L. Paddock, S.H. Rongey, P.H. McPherson, A. Juth, G. Feher and M.Y. Okamura; Biochemistry 33, 734-745 (1994).
76. Protonation and Free Energy Changes Associated with Formation of QB H2 in Native and Glu-L212 to Gln Mutant Reaction Centers from Rhodobacter sphaeroides; P.H. McPherson, M. Schönfeld, M. L. Paddock, M.Y. Okamura and G. Feher; Biochemistry 33, 1181-1193 (1994).
77. Proton-Coupled Electron Transfer Reactions of QB in Reaction Centers from Photosynthetic Bacteria; M.Y. Okamura and G. Feher; in Anoxygenic Photosynthetic Bacteria (1995), edited by R. E. Blankenship, M. T. Madigan and C. E. Bauer (Kluwer Academic Publishers), pp. 577-593.
78. Direct Charge Recombination from D+QA QA-· to DQAQB in Bacterial Reaction Centers from Rhodobacter sphaeroides ; A. Labahn, M. L. Paddock, P.H. McPherson, M.Y. Okamura and G. Feher; J. Phys. Chem. 98, 3417-3423 (1994).
79. Electrogenic Events in Bacterial Reaction Centers in a Lipid Monolayer; I. Characterization of the System; P. Brzezinski, Y. Blatt, A. Gopher, D. Kleinfeld, A. Messinger, M. Montal, M. Y. Okamura and G. Feher; Biophysical J. (submitted 10/30/93).
80. Direct Charge Recombination from D+QAQB-· to DQAQB in Bacterial Reaction Centers from Rhodobacter sphaeroides Containing Low Potential Quinone in the QA Site; A. Labahn, J. M. Bruce, M. Y. Okamura and G. Feher; Chemical Physics 197, 355-366 (1995).
81. Electrostatic Calculations of Amino Acid Titration and Electron Transfer, QA-·QB to QAQB-· , in the Reaction Center; P. Beroza, D. R. Fredkin, M. Y. Okamura and G. Feher; Biophysical J., 68, 2233-2250 (1995).
82. Pathway of Proton Transfer in Bacterial Reaction Centers: Further Investigations on the Role of SER- L223 Studied by Site-Directed Mutagenesis; M. L. Paddock, G. Feher and M. Y. Okamura; Biochemistry 34, 15742-15750 (1995).
83. Investigation of the Binding Domain in the Rhodobacter sphaeroides Cyt C2 :Reaction Center Complex by Site-Directed Mutagenesis of Asp-M184 and Asp-L155 to Lys; S. H. Rongey, G. Feher and M. Y. Okamura; (Proceedings of the Xth International Photosynthesis Congress, in Montpellier, France, August 20-25, 1995).
84. Suppressor Mutation in the H-Subunit Partially Restores Proton Transfer in Asp-L213 to Asn Mutant Reaction Centers from Rhodobacter sphaeroides; M. E. Senft, M. L. Paddock, S. H. Rongey, G. Feher and M. Y. Okamura; in Photosynthesis: from Light to Biosphere, Vol. 1, pp. 639-642 (Proceedings of the Xth International Photosynthesis Congress, in Montpellier, France, August 20-25, 1995, Ed. P. Mathis), Kluwer Academic Publishers, Dordrecht, 1995.
85. Protonation of Glu L212 Following QB-· Formation in the Photosynthetic Reaction Center of Rhodobacter sphaeroides: Direct Evidence from Time-Resolved Infrared Spectroscopy; R. Hienerwadel, S. Grzybek, C. Fogel, W. Kreutz, M.Y. Okamura, M.L. Paddock, J. Breton, E. Nabedryk and W. Mäntele; Biochemistry 34, 2832-2843 (1995).
86. Fourier Transform Infrared Difference Spectroscopy of Secondary Quinone Acceptor Photo reduction in Proton Transfer Mutants of Rhodobacter sphaeroides; N. Nabedryk, J. Breton, R. Hienerwadel, C. Fogel, W. Mäntele, M.L. Paddock and M.Y. Okamura; Biochemistry 34, 14722-14732 (1995).
87. Co-Crystallization and Characterization of the Photosynthetic Reaction Center-Cytochrome c2 Complex from Rhodobacter sphaeroides; N. Adir, H.L. Axelrod, P. Beroza, R.A. Isaacson, S.H. Rongey, M.Y. Okamura and G. Feher; Biochemistry 35, 2535-2547 (1996).
88. Mechanism of Proton-Coupled Electron Transfer for Quinone (QB) Reduction in Reaction Centers of Rb. sphaeroides; M. S. Graige, M. L. Paddock, J. M Bruce, G. Feher and M. Y. Okamura; J. Am. Chem. Soc. 118, 9005-9016 (1996).
89. Light-Induced Electrogenic Events
Associated with Proton Uptake Upon Forming QB-· in Bacterial Wild-Type and
90. Proton and Electron Transfer to the Secondary Quinone (QB) in Bacterial Reaction Centers: The Effect of changing the Electrostatics in the Vicinity of QB by interchanging Asp and Glu at the L212 and L213 Sites; M. L. Paddock, G. Feher and M. Y. Okamura; Biochemistry 36, 14238-14249 (1997).
91. Characterization of Second Site Mutations Show that Fast Proton Transfer to QB-· is Restored in Bacterial Reaction Centers of Rhodobacter sphaeroides Containing the Asp-L213 to Asn Lesion; M. L. Paddock, M. E. Senft, M. S. Graige, S. H. Rongey, T. Turanchik, G. Feher and M. Y. Okamura; Photosynthesis Research 55, 281-291 (1998).
92. Identification of Proton Transfer Pathways in the X-Ray Crystal Structure of the Bacterial Reaction Center from Rhodobacter sphaeroides; E. C. Abresch, M. L. Paddock, M. H. B. Stowell, T. M. McPhillips, H. L. Axelrod, S. M. Soltis, D. C. Rees, M. Y. Okamura and G. Feher; Photosynthesis Research 55, 119-125 (1998).
93. Free Energy Dependence of the Direct Charge Recombination from the Primary and Secondary Quinones in Reaction Centers from Rhodobacter sphaeroides; J. P. Allen, J. C. Williams, M. Graige, M. L. Paddock, A. Labahn, G. Feher and M. Y. Okamura; Photosynthesis Research 55, 227-233 (1998).
94. Direct Evidence of Structural Changes in Reaction Centers of Rb. sphaeroides Containing Suppressor Mutations for Asp L213 → Asn: A FTIR Study of QB Photoreduction; Eliane Nabedryk, Jacques Breton, Melvin Y. Okamura and Mark L. Paddock; Photosynthesis Research 55, 293-299 (1998).
95. A puhA Gene Deletion and Plasmid Complementation System for Facile Site-Directed Mutagenesis Studies of Reaction Center H Protein of Rhodobacter sphaeroides; X-Y Chen, V. Yurkov, M.L. Paddock, M.Y. Okamura and J.T. Beatty; Photosynthesis Research 55, 369-373 (1998).
96. Conformation Gating of the Electron Transfer Reaction QA-·QB to QAQB-·, in RCs of Rb. sphaeroides Determined by a Driving Force Assay; M. S. Graige, G. Feher and M. Y. Okamura; Proc. Natl. Acad. Sci. USA95, 11679-11684 (1998).
97. Proton Uptake by Carboxylic Acid Groups upon Photoreduction of the Secondary Quinone (QB) in Bacterial Reaction Centers from Rhodobacter Sphaeroides: FTIR Studies on the Effects of Replacing Glu H173; E. Nabedryk, J. Breton, M.Y. Okamura and M.L. Paddock; Biochemistry 37, 14457-14462 (1998).
98. Temperature Dependence of the Direct Charge Recombination from QB-· to D+ in Bacterial Reaction Centers of Rhodobacter sphaeroides; A. Labahn, R. Schmid, M. Y. Okamura and G. Feher; in: Photosynthesis: Mechanisms and Effects, Vol. II , G. Garab, Ed; Kluwer Academic Publishers, Netherlands, pp. 829- 832 (1998).
99. The Primary and Secondary Acceptors in Bacterial Photosynthesis: II The Structure of the Fe2+ - Q-· complex; G. Feher and M. Y. Okamura; Appl. Magn. Reson., 16, 63-100 (1999).
100. Identification of the Proton Pathway in Bacterial Reaction Centers: Inhibition of Proton Transfer by Binding of Zn2+ or Cd2+; M. L. Paddock, M. S. Graige, G. Feher & M. Y. Okamura; Proc. Natl. Acad. Sci. USA 96,6183-6188 (1999).
101. Observation of the Protonated Semiquinone Intermediate in Isolated Reaction Centers from Rb. sphaeroides: Implications for the Mechanism of Electron & Proton Transfer in Proteins; M. S. Graige, M. L. Paddock, G. Feher, and M. Y. Okamura: Biochemistry, 38,11465-11473 (1999).
102. Determination of the Binding Sites of the Proton Transfer Inhibitors Cd2+ and Zn2+ in Bacterial Reaction Centers; H.L. Axelrod, E.C. Abresch, M.L. Paddock, M.Y. Okamura and G. Feher: Proc. Natl. Acad. Sci., USA 97, 1542-1547 (2000).
103. Identification of the Proton Pathway in Bacterial Reaction Centers: Replacement of Asp-M17 and Asp-L210 with Asn Reduces the Proton Transfer Rate in the Presence of Cd2+; M.L. Paddock, G. Feher and M.Y. Okamura, Proc. Natl. Acad. Sci., USA 97, 1548-1553 (2000).
104. Proton and Electron Transfer in
105. EPR Study of the Molecular and Electronic Stucture of the Semiquinone Biradical QA-· QB-· in Photosynthetic Reaction Centers from Rb. sphaeroides; R. Calvo, E.C. Abresch, R. Bittl, G. Feher, W. Hofbauer, R.A. Isaacson, W. Lubitz, M.Y. Okamura, and M.L. Paddock; J. Am. Chem. Soc. 122, 7327-7341 (2000).
106. Identification of the Proton Pathway in Bacterial Reaction Centers: Both Protons Associated with Reduction of QB to QBH2 Share a Common Entry Point; P. Ädelroth, M.L. Paddock, L.B. Sagle, G. Feher and M.Y. Okamura; Proc. Natl. Acad. Sci., USA, 97, 13086-13091 (2000).
107. EPR Study of the Semiquinone Biradical QA-· QB-· in Photosynthetic Reaction Centers from Rb. Sphaeroides at 326 GHz: Determination of the Exchange Interaction J0; R. Calvo, R. Isaacson, M.L. Paddock, E.C. Abresch, M.Y. Okamura, A.-L. Maniero, L.-C. Brunel and G. Feher; Journal of Physical Chemistry B, 105, 4053-4057 (2001).
108. Interaction between Cytochrome c2
and the
109. Identification of the Proton Pathway in Bacterial Reaction Centers: Cooperation between Asp-M17 and Asp-L210 Facilitates Proton Transfer to the Secondary Quinone (QB); M.L. Paddock, P. Adelroth, C. Chang, E.C. Abresch, G. Feher and M.Y. Okamura; Biochemistry, 40, 6893-6902 (2001).
110. Identification of the Proton Pathway in Bacterial Reaction Centers: Decrease of Proton Transfer Rate by Mutation of surface Histidines at H126 and H128 and Chemical Rescue by Imidazole Identifies the Initial Proton Donors; P. Ädelroth, M.L. Paddock, A. Tehrani, J.T. Beatty, G. Feher and M.Y. Okamura; Biochemistry, 40, 14538-14546 (2001).
111. Identification of the Proton Pathway in Bacterial Reaction Centers: Decrease of Proton Transfer Rate by Mutation of surface Histidines at H126 and H128 and Chemical Rescue by Imidazole Identifies the Initial Proton Donors; P. Ädelroth, M.L. Paddock, A. Tehrani, J.T. Beatty, G. Feher and M.Y. Okamura; Biochemistry, 40(48), 14538-14546 (2001).
112. Identification of Electrostatic Interactions Important for Docking Cytochrome c2 onto the Bacterial Reaction Center by Double Mutant Studies; M.Tetreault, M. Cusanovich, T. Meyer, H.L. Axelrod and M.Y. Okamura; Biochemistry, accepted for publication (2002).
113. X-Ray Structure Determination of the Cytochrome c2:Reaction Center Electron Transfer Complex from Rhodobacter sphaeroides; H.L. Axelrod, E.C. Abresch, M.Y. Okamura, A.P. Yeh, D.C. Rees, G. Feher; J. Mol. Biol., 319, 501-515 (2002).
114. Rapid-scan Fourier Transfor Infrared Spectroscopy shows Coupling of Glu-L212 Protonation and Electron to QB in Rhodobacter sphaeroides Reaction Centers; A. Mezzetti, E. Nabedryk, J. Breton, M.Y. Okamura, M.L. Paddock, G. Giacomett, W. Leible; BBA – Bioenergetics, 1553, 320-330 (2002).
115. Determination of Proton Transfer Rates by Chemical Rescue: Application to Bacterial Reaction Centers; M.L Paddock, P. Ädelroth, G. Feher, M.Y. Okamura, J. Thomas Beatty; Biochemistry, 41, 14716-25 (2002).
116. Double Mutant Studies Identify Electrostatic Interactions that are
Important for Docking Cytochrome c2 onto
the
117. Spin-lattice relaxation of coupled metal-radical spin-dimers in proteins: application to Fe2+-cofactor (QA-·, QB-·, f-·) dimers in reaction centers from photosynthetic bacteria; R. Calvo, R.A. Isaacson, E.C. Abresch, M.Y. Okamura, G. Feher; Biophys. J., 83, 2440-2456 (2002).
118. The Mechanism of Proton Transfer Inhibition by Cd2+
Binding to Bacterial Reaction Centers: Determination of the PKA of
Functionally Important HIS Residues; M.L. Paddock, L. Sagle, A. Tehrani, J.T. Beatty, G. Feher and M.Y. Okamura; Biochemistry, in print (2003).
119. Proton Transfer Pathways and Mechanism in Bacterial Reaction Centers ; M.L. Paddock, G. Feher, M.Y. Okamura; Febs Lett. 555, 45-50 (2003).
120. X-Ray Structure Determination of Three Mutants of the Bacterial
Photosynthetic Reaction Centers from Rhodobacter sphaeroides: Altered Proton Transfer Pathways; Q. Xu, H.L. Axelrod, E.C. Abresch, M.L. Paddock, M.Y. Okamura, G. Feher; Structure, submitted (2004).